Skin-to-Brain Stem Cell Technology

Project Outlines and Aims

Synaptogenesis and neurogenesis are thought to be important processes mediating the clinical expression of dementia. Our group is therefore investigating the possibility of exogenously augmenting synaptic and neural connections by neural stem cell (NSC) transplantation (cell replacement therapy). Specifically, Dr Valenzuela has brought together a group to pursue the objective of efficiently deriving NSCs from skin. This strategy benefits from the major clinical advantage that autogenic source tissue can be readily obtained.

We receive skin samples from canines undergoing routine veterinary care. These skin samples are processed and then placed in the neurosphere assay for the formation of heterogeneous neural precursor colonies. These are then transferred to a secondary assay in order to increase the homogenous proliferation of skin-derived NSCs.

We have successfully induced NSC-like cells from the skin of four different dogs. PCR characterization found positive nestin, CD133 and NCAM expression, similar to NSCs derived from fetal brain tissue. Further characterization, clonal analysis and differentiation studies are ongoing. Should we succeed with our aims, significant new intellectual property is expected. This technology is planned to eventually be applied in a trial of the treatment of Canine Cognitive Dysfunction.

Staff

Dr Michael Valenzuela (chief investigator)
Dr Sophia Dean (post doctoral fellow)
Lawrence Choi (research assistant)
Professor Perminder Sachdev

Collaborators

Associate Professor Kuldip Sidhu, Prince of Wales Chief Stem Cell scientist, Diabetes Transplant Unit